for his work on ribonucleaseandfor their contribution to the understanding of the connection between chemical structure and catalytic activity of the ribonuclease molecule
for their elucidation of the enzymatic mechanism underlying the synthesis of adenosine triphosphateandfor his discovery of an ion-transporting enzyme, Na+, K+ -ATPase
... was Holy Roman Emperor (as Charles V) from 1519-1558; he was also King of Spain from 1516-1556 -- officially as Charles I of Spain, although often referred to as Charles ...