The existence of these sounds was not suspected for quite some time, because Hittite and the Anatolian languages are the only Indo-European languages in which they ever survive as actual phonemes that appear in the records we have of those extinct languages. That said, the existence of these sounds is now accepted by most philologists, because positing their existence simplifies some otherwise hard to explain sound changes that appear in the descendant languages of PIE.
There were three such laryngeals:
In Greek, between consonants h1 > e, h2 > a, and h3 > o. In Indo-Iranian languages such as Sanskrit, each laryngeal becomes i, and in all other Indo-European languages, each laryngeal becomes a. This explains such observed phenomena as:
The chief evidence of laryngeals was the fact that when they appeared in connection with the PIE vowel *e-, h2 coloured it to *a-, and h3 to *o-. In Anatolian, however, h2 was preserved, and h3 was preserved in certain positions. For example:
The laryngeal theory has been posited as the best explanation of the otherwise mysterious appearance of h- in the Anatolian words, and the vowel difference between the Anatolian languages and most other Indo-European languages, such as Latin ovis.
The laryngeal theory requires fairly widespread adjustments in our view of the inflections of Indo-European. We now know that PIE may have had two original grammatical genders, masculine and neuter. The feminine gender that most of the oldest Indo-European languages share may have been formed by a suffix, *-eh2, which was coloured by the laryngeal to *-a. This makes the feminine nouns and adjectives originally consonant stems rather than original vowel stems, and helps explain why they are inflected differently from other nouns that are true vowel stems.
The laryngeal theory also explains a number of different ablaut sequences that appear in many Indo-European roots, and makes them seem less arbitrary and more regular. For example, the observed sequences:
Considerable debate still surrounds the pronunciation of the laryngeals. Many believe that they represented some sort of glottal stop or pharyngeal; others assume that *h2 was a velar and *h3 was a labiovelar fricative. Further still, others hold that they had no pronunciation at all, and are simply phonetic coefficients.
Robert S. P. Beekes: Comparative Indo-European Linguistics (John Benjamins, 1995) ISBN 1-55619-505-2
Indo-European phonology: http://www.tundria.com/Linguistics/IEPhonol.htm
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