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SDS-PAGE
SDS-PAGE stands for Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis. It is a technique used in biochemistry and molecular biology to separate proteins according to their size/molecular weight.
Picture of an SDS-PAGE. The molecular marker is in the left lane.
The proteins are inserted into a (flat) gel and an electrical current is applied. The proteins will move trhough the gel. The SDS is added to the gel mixture to denaturate the proteins, to unfold them so they will not move through the gel by their folding or electrical charge, but their size. Different proteins will form distinct bands within the gel. The parallel electrophoresis of proteins or protein mixes in different lanes on the same gel makes it possible to determine protein sizes by comparing their bands to those of proteins of known size.
Picture of an SDS-PAGE. The molecular marker is in the left lane.
- See also : Western blot
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