Encyclopedia > SDS-PAGE

  Article Content

SDS-PAGE

SDS-PAGE stands for Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis. It is a technique used in biochemistry and molecular biology to separate proteins according to their size/molecular weight.

The proteins are inserted into a (flat) gel and an electrical current is applied. The proteins will move trhough the gel. The SDS is added to the gel mixture to denaturate the proteins, to unfold them so they will not move through the gel by their folding or electrical charge, but their size. Different proteins will form distinct bands within the gel. The parallel electrophoresis of proteins or protein mixes in different lanes on the same gel makes it possible to determine protein sizes by comparing their bands to those of proteins of known size.


Picture of an SDS-PAGE. The molecular marker is in the left lane.

See also : Western blot



All Wikipedia text is available under the terms of the GNU Free Documentation License

 
  Search Encyclopedia

Search over one million articles, find something about almost anything!
 
 
  
  Featured Article
Reformed churches

... churches in Nigeria (and founding counterparts) 1 International organizations of Reformed churches Continental Reformed churches Hungarian Reformed Church[?] Th ...

 
 
 
This page was created in 24.4 ms