DNA ligase can ligate broken DNA strands, forming covalent phosphodiester bonds.
An example of how a ligase works (with sticky ends):
5'-AGTCTGATCTGACT GATGCGTATGCTAGTGCT-3' 3'-TCAGACTAGACTGACTACG CATACGATCACGA-5' becomes 5'-AGTCTGATCTGACTGATGCGTATGCTAGTGCT-3' 3'-TCAGACTAGACTGACTACGCATACGATCACGA-5'
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